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Fig. 1. Primary structure of the urea transporter (UT) isolated from the kidney of
Bufo marinus. (A) The deduced amino acid sequence is aligned with
those of Rana esculenta UT (GenBank accession no. Y12784), rat UT-A2
(U09957) and rat UT-B2 (U81518) using the Clustal algorithm. Asterisks denote
identical amino acid residues; the horizontal bars indicate the predicted
transmembrane regions; the box indicates putative N-glycosylation
sites; the underline indicates the ALE domain, which is considered to be a
signature sequence for UT-B. (B) Kyte-Doolittle hydropathy profile of the
deduced Bufo UT amino acid sequence predicts the presence of
trans-membrane regions (1–10).
