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Fig. 8. Dopamine stimulation causes secretory vesicle loss and appearance of
vacuoles. Cyprids were fixed at different times during exposure to dopamine (1
mmol l1) and sectioned. Sections were stained with Toluidine
Blue, and the different types of vesicles in the stained sections were counted
under the microscope. Note that dense-core granules (type 1) reduce in number
over time, with a proportionate increase in the number of vacuoles (type 4)
after 10 min of dopamine treatment.