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Fig. 4. Metabolite changes under control conditions. Information from N=282 intervals of 25 min duration from all five animals. Intervals were grouped according to change in inorganic phosphate between successive NMR spectra. Groups were: [P_i]=0–0.99 µmol g^–1 wet mass (N=88 cases), [P_i]=1–1.99 µmol g^–1 wet mass (N=25 cases), [P_i]=2–2.99 µmol g^–1 wet mass (N=9 cases) and [P_i]=>3 µmol g^–1 wet mass (N=9 cases) on the positive side; [P_i]=0 to –0.99 µmol g^–1 wet mass (N=90 cases), [P_i]=–1 to –1.99 µmol g^–1 wet mass (N=31 cases), [P_i]=–2 to –2.99 µmol g^–1 wet mass (N=18 cases) and [P_i]=<–3 µmol g^–1 wet mass (N=9 cases) on the negative side. Number of cases per group (displayed in B) are a direct measure of the probability of occurrence, as all intervals were picked randomly. Linear regression equations: (A) pH_i=7.444+0.022[P_i]; r² =0.96, F_1,5=121, P<0.001 (for the range <–3 to 3 µmol g^–1 wet mass); (B) [PLA]_i=–0.88[P_i]; r² =0.99, F_1,6=222, P<0.001; (C) H⁺_i=–0.41–1.58[P_i]; r²=0.94, F_1,5=88, P<0.001 (for the range <–3 to 3 µmol g^–1 wet mass); (D) For ANOVA, see text. All concentrations in µmol g^–1 wet mass (=wet mass^–1).

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