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Fig. 1. (A) Animal in chamber in front of the magnet, connected to a seawater
perfusion system. Sliders restrict the space available to the animal to a
minimum. A grey box indicates the position of the 31P surface coil
below the animal's thick ventral mantle muscle. The pressure catheter (not
visible) leaves the animal's mantle cavity and passes through the lid on the
right towards a pressure transducer (see text). (B) Schematic illustration of
the surface coil and its sensitive volume (grey semi-circle). Metabolite
changes within radial and circular muscles of the mantle muscle organ can be
recorded. Circular muscle consists of a central bulk of anaerobic fibres and
two thin layers of outer, aerobic fibres. (C) Schematic illustration of
intervals defined between the acquisition of successive in vivo NMR
spectra. One complete 25 min interval is illustrated (n), which is divided
into 12 segments (s1n–s12n). For each segment,
swimming jet (SJ) pressure amplitudes and frequencies were determined,
allowing us to calculate jet indices (JIs) for variable time intervals (by
summing up segment JI within intervals a to l; e.g. interval g consists of JIs
from segments s6n–s12n). For further explanations,
see text.
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