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Fig. 7. BCF in toads in the dry chamber (filled columns) and in the wet chamber
(open columns). Each column represents mean ± 1 s.e.m. of 15 trials.
(A) In B. alvarius BCF did not increase when toads with ad
libitum bladder water were placed on water (group A). Removing the
bladder depot resulted in a significant stimulation of BCF in the wet chamber
(group B). Mild dehydration resulted in a similar increase in BCF following
water contact (group C). Dehydration (mean 15%) caused a further increase in
BCF (group D). (B) B. marinus voided their bladders so ad
libitum bladder measurements could not be obtained. Empty bladder B.
marinus did not increase BCF when placed in on water (group B). Mild
dehydration produced a small but significant increase in BCF (group C).
Dehydration (16.9%) caused a further significant increase in BCF on water
(group D). On dry substrate BCF did not increase significantly with
dehydration in any of the species. **P<0.01;
***P<0.001.