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Figure 1


Fig. 1 (A) Trace from the flow cytometer showing the increase in seat patch BCF of a B. alvarius dehydrated by 9.2% and placed on DI at time 0. (B) Mean seat patch BCF values ± s.e.m. from 15 trials (three replicates in five toads) with dehydrated B. alvarius; open symbols, BCF in the dry chamber; filled symbols, BCF on DI. (C) Effect of handling on BCF. Open symbol, BCF prior to handling; repositioning the toads on the probe caused a rapid decline in BCF, when the toads were left undisturbed BCF increased to the prehandling level within 3 min (filled symbols). The increase in BCF by dehydrated toads with 50 mmol l–1 NaCl in the reservoir was not different from that in A and B. P, pre-handling level.





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