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Figure 4


Fig. 4. Temora stylifera female copepods. (A,C,E,G) Reconstructed three-dimensional confocal laser scanning microscopy images obtained using both the 488 and 543 nm {lambda} lasers to excite fluorescein and chlorophyll, respectively. (B,D,F,H) The same copepods as in A,C,E,G, seen in transmitted light. (A,B) A starved female (time=0). In A the chitinous wall is autofluorescent, the yellow colour is the result of superimposition of green and red fluorescence. (C,D) A female fed the dinoflagellate Prorocentrum minimum for 48 h. In C the red fluorescence inside the gut is due to chlorophyll emission. (E,F) A female fed FitcDx-encapsulating liposomes (LIPOF) for 48 h. In E the green fluorescence inside the gut is due to the emission of FitcDx. (G,H) Fluorescent 3-D image of a female fed a mixed diet of LIPOF and P. minimum for 24 h. In G the green fluorescence in the gut does not overlap with the red fluorescence of chlorophyll. Bar, 0.2 mm.





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