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Fig. 1. RT-PCR amplification of the 3' ends of mRNAs that encode Kv4 and
KChIP, extracted from heart tissue of C. intestinalis. (A) Fragments
of 
1500 bp corresponding to the 3' end of the transcript for a Kv4
channel, amplified by 3'-RACE. No cDNA was added to the control
reaction. (B) Diagram showing the organization of the CionaKv4 gene,
based on an alignment between the transcript sequence for CionaKv4
and scaffold 168 of the genomic database for C. intestinalis (release
version 1.0). Exons are shown as black boxes and numbered in Arabic numerals
(0-6). Introns are shown as white boxes and numbered in Roman numerals (I-VI).
Approximate intron sizes (in bp) are indicated above the introns. Exon sizes
(in bp) are indicated below the boxes. Exon sizes, but not intron sizes, are
drawn approximately to scale. The 3'-UTR region is represented by a box
shaded in grey. The box representing the 5'-UTR region is delimited by a
discontinuous line to indicate that the sequence of this region was not
determined in the present study. (C) Fragments of 600 bp, corresponding
to the 3' end of the transcript for a KChIP subunit, amplified by
3'-RACE. No cDNA was added to the control reaction. (D) Diagram showing
the exon/intron structure of the CionaKChIP gene, as derived from an
alignment between the sequence of the CionaKChIP transcript and
scaffold 457 of the genomic database for C. intestinalis (release
version 1.0). Exons are shown as black boxes and numbered in Arabic numerals
(1-7). Introns are shown as white boxes and numbered in Roman numerals (I-VI).
Intron and exon sizes (in bp) are indicated, respectively, above or below the
diagram and are drawn approximately to scale. The 3'-UTR region is
represented by box shaded in grey. The 5'-UTR region is delimited by a
discontinuous line to indicate that its sequence was not determined in this
study.
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