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Fig. 2. Operantly conditioned semi-intact preparations demonstrated a reduction in
total breathing following conditioning for learning and LTM. Conditioned and
yoked control preparations were given a 20 min pre-observation period and a 20
min post observation period to assess total breathing time before and after
their operant and yoked sessions, respectively. During the pre- and
post-observation periods both conditioned and yoked preparations were allowed
to breathe freely. The total breathing time of conditioned and yoked
preparations during the pre-observation period did not significantly differ
from each other (unpaired t-test=0.4451, P>0.05). Only
the conditioned preparations demonstrated a significant reduction in total
breathing time during the post observation period, which followed the LTM test
(paired t-test=3.090, P=0.01).
**P=0.01.
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