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Fig. 5. Turnover numbers (kcat) of rWT M. trossulus
cMDH and the mutants V114N and V114H from 5 to 35°C. Mutations V114N and
V114H significantly reduce the catalytic rate relative to rWT at all
temperatures except 5°C for V114H. Solid bars indicate the physiological
immersion temperature ranges of M. trossulus and M.
galloprovincialis, where rWT and V114N exhibit similar catalytic
rates.