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Figure 3


Fig. 3. Michaelis-Menten constants for the cofactor NADH of cMDHs either purified from muscle tissue or expressed recombinantly. (A) KmNADH values for cMDHs purified from muscle tissue of M. trossulus, M. galloprovincialis and M. californianus, measured from 5 to 45°C [* indicates a significant difference ({alpha}=0.05) between M. trossulus and M. galloprovincialis cMDHs; ** between M. trossulus and M. californianus; and *** between M. californianus and M. galloprovincialis]. (B) KmNADH values of recombinant M. trossulus cMDH (rWT) and mutants of M. trossulus cMDH replacing the residue at position 114 with that found in M. galloprovincialis (V114N) or M. californianus (V114H), measured from 5 to 45°C [* indicates a significant difference ({alpha}=0.05) between rWT and V114N cMDHs, ** between rWT and V114H; and *** between V114H and V114N]. (C) A comparison of KmNADH values measured at 35°C between M. trossulus (M. t.) cMDH and rWT, M. galloprovincialis (M. g.) cMDH and mutant V114N, and M. californianus (M. c.) cMDH and mutant V114H. KmNADH values sharing a letter designation (X,Y,Z) are not significantly different ({alpha}=0.05); all other comparisons are significantly different.





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