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Figure 6


Fig. 6. A pH-[HCO3-] diagram for rainbow trout at 13°C. The PCO2 for a given combination of pH and [HCO3-] was calculated using the Henderson-Hasselbalch equation and the appropriate values for pK' and the solubility coefficient {alpha}CO2 (Boutilier et al., 1984). The buffer line for rainbow trout true plasma was constructed using buffer values derived by Wood et al. (1982). The labelled points represent mean data (±1 s.e.m., N=8) for (1) pre-exposure, (2) pre-acetazolamide injection and (3) post-acetazolamide injection sampling points for trout exposed to hypercarbia (nominal PWCO2 of 6 Torr; 1 Torr=133.3 Pa).





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