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Figure 5


Fig. 5. The effects of exposure to hypercarbia (nominal PWCO2=6 Torr; 1 Torr=133.3 Pa) and acetazolamide treatment (30 mg kg-1) on branchial (A) net excretion of acidic equivalents (JnetH+), (B) titratable net acid flux (JnetTA) and (C) net ammonia excretion (JnetNH3) in rainbow trout (Oncorhynchus mykiss). JnetH+ was calculated as the sum of JnetTA and JnetNH3, signs considered. Data are mean values ± 1 s.e.m.; N=11 for both control and hypercarbia treatment groups. Data were analyzed by two-way RM ANOVA with treatment group (control versus hypercarbic) and sampling time [pre- or post-acetazolamide (Az) injection] as factors; P values are indicated on the figure. An asterisk (*) indicates a significant difference as a result of Az injection within a treatment group, while a dagger ({dagger}) indicates a significant difference between control and hypercarbia treatment groups within a given sampling time. Where significant interactions did not occur, only the P values are indicated on the figure.





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