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Figure 2


Fig. 2. Effects of surgery on spontaneous behaviour. (A) All spontaneous behaviours observed, divided into experimental groups. See Materials and methods for a description of the surgical treatments and Table 1 for descriptions of the behaviours. The control group (N=40) included both intact leeches and sham-operated controls; we found no differences between the two groups. Nerve cut, N=9. Ablated some or all of the SubEG, N=33. In Figs 2, 3, 4, 5, 6, * indicates locomotory behaviours, which are discussed in Fig. 7. (B) Distribution of behaviours along the CCA1 behaviour score axis. The behaviours that lie furthest to the left or right along this axis most strongly distinguished among treatment groups. Behaviours located in the centre of the axis were seen in all groups. (C) Distributions of individual CCA1 scores in the three experimental groups. Individual scores within each group were binned and arrayed along the CCA1 axis. Crawling and probing distinctively characterized the control group, whereas swimming and incomplete crawling (ic) characterized leeches with ablations.





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