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Fig. 2. Effects of surgery on spontaneous behaviour. (A) All spontaneous behaviours
observed, divided into experimental groups. See Materials and methods for a
description of the surgical treatments and
Table 1 for descriptions of the
behaviours. The control group (N=40) included both intact leeches and
sham-operated controls; we found no differences between the two groups. Nerve
cut, N=9. Ablated some or all of the SubEG, N=33. In Figs 2,
3,
4,
5,
6, * indicates
locomotory behaviours, which are discussed in
Fig. 7. (B) Distribution of
behaviours along the CCA1 behaviour score axis. The behaviours that lie
furthest to the left or right along this axis most strongly distinguished
among treatment groups. Behaviours located in the centre of the axis were seen
in all groups. (C) Distributions of individual CCA1 scores in the three
experimental groups. Individual scores within each group were binned and
arrayed along the CCA1 axis. Crawling and probing distinctively characterized
the control group, whereas swimming and incomplete crawling (ic)
characterized leeches with ablations.