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Fig. 4. The effect of rapid cold-hardening (RCH) on cellular survival of various
tissues from cold-acclimated larvae of Belgica antarctica. Green
indicates living cells, and red indicates dead cells. Scale bar, 300 µm for
rapidly cold-hardened gut and Malpighian tubules, and Malpighian tubules
frozen with no prior treatment; 150 µm for all other tissues.