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Fig. 2. Effect of rapid cold-hardening on the freeze tolerance of (A)
summer-acclimatized larvae and (B) adults of the Antarctic midge, Belgica
antarctica. Cultures were maintained at 4°C prior to exposure to the
indicated temperatures. * denotes a significant
(P<0.05) difference in survival between groups directly exposed to
-10°C and those that were first held at -5°C for 1 h prior to the
-10°C exposure. Values are means ± s.e.m. based on six replicates
of 10 individuals.
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