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Fig. 4. Partially melanized L. vannamei male, and dissected components of
the distal part of its reproductive system. (A) Ventral view of the intact
male. Symmetrical dark brown melanin spots are clearly visible through the
cuticle near the genital papillae. (B) The dissected ampoules with intact
spermatophores. Here, the muscular ampoules with their intact spermatophores
are stretched and turgid. (C) The same ampoules (top) after the spermatophores
(bottom) had been dislodged from them by applying a gentle pressure on the
edge of the ampoules opposite to the gonopore, mimicking ejaculation. The
empty ampoules then lost their turgidity and became flaccid and slack. Note
the melanized flake that separated out from the right spermatophore
(arrowhead). Scale bars, 5 mm (A,B), 3 mm (C).