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Figure 7


Fig. 7. Inhibition of laminarin-mediated H2O2 generation in haemocytes. The production of H2O2 was investigated in haemocytes pre-incubated with the PKC inhibitors (A) GF109203X (0.01-10 µmol l-1) and (B) Gö 6976 (0.01-10 µmol l-1), or (C) the NADPH oxidase inhibitor apocynin (10-500 µmol l-1), for 30 min prior to stimulation with laminarin (10 mg ml-1). Values are means ± s.d. of two independent experiments each performed in triplicate. *P≤0.05, **P≤0.01 and ***P≤0.001 when compared to H2O2 production in stimulated haemocytes not exposed to either inhibitor (shown as 100%, broken line).





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