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Figure 5


Fig. 5. PKC phosphorylation is PI-3-K-independent but phospholipase C-dependent in laminarin-stimulated haemocytes. Haemocyte monolayers were pre-treated for 30 min with the phospholipase C inhibitors U-73122 and ET-18-OCH3 or the PI-3-K inhibitor LY294002 at similar range of concentrations (0.001-10 µmol l-1), or vehicle prior to the addition of laminarin (10 mg ml-1) for 10 min. Phosphorylated PKC was detected by immunoblotting with the anti-phospho PKC (pan) antibody. (A) For U-73122 inhibition studies, immunoblots are representative of four independent experiments. In the case of (B) ET-18-OCH3 and (C) LY294002 inhibition studies, immunoblots are representative of three independent experiments.





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