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Fig. 3. The PKC inhibitor, calphostin C reduces MEK and ERK 1/2 phosphorylation in
laminarin-challenged haemocytes. Haemocyte monolayers were incubated with
calphostin C (0.001-10 µmol l-1), a PKC inhibitor that interacts
with the cysteine-rich zinc finger structure of the PKC regulatory domain, or
vehicle (0.1% DMSO), prior challenge with laminarin (10 mg ml-1)
for 10 min. Protein extracts were subjected to SDS-PAGE followed by western
blotting using polyclonal phospho-specific antibodies to (A) MEK and to (B)
ERK. Anti-actin antibodies were used to confirm equal loading of proteins.
Immunoblots are representative of two independent experiments.
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