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Fig. 5. Discrimination of the M-spike with extracellular recordings in caudal
hindbrain. (A) Schematic representation of the experimental arrangement,
superimposed drawings of the M-cells onto the medulla oblongata with
simultaneous paired intra- and extracellular recording were used for B-E. CB,
cerebellum; VII, facial lobe; X, vagal lobes (B,Ci,Cii) Extracellular
responses recorded close to the left M-axon. Stimulus intensity was gradually
increased from subthreshold for both M-axons (black), to above threshold for
one (blue) or both (red). Asterisk indicates M-spike(s). (Ci,Cii) Amplitudes
of the surface M-spike fields in B as functions or recording distance from the
midline of the caudal hindbrain (Ci) and the amplitude of the single spike at
the midline as a function of depth from the surface (Cii). Negative depths are
in the saline superfusate. (D) Hindbrain field potentials evoked by
orthodromic M-cell activation. Suprathreshold current injection in the left
M-cell soma (top), and the corresponding M-axon spike and compound action
potentials in the hindbrain (middle). After moving the intracellular electrode
to the left M-soma (not shown), the directly activated left M-axon spike and
compound field potentials was recorded instead (asterisk) (bottom). Asterisk
and horizontal broken line indicate M-axon spike and M-triggered activity,
respectively. (E) Compound fields correlated with sound evoked M-cell spike
activity. The sound stimulus (top) evoked an EPSP recorded from the M-soma
before (red) and after (blue) Cl- injection; (bottom) corresponding
fields recorded in the hindbrain. Note the two M-spikes (asterisks) are
separated temporally by M-triggered activity (broken line).
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