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Figure 5


Fig. 5. Expression of sea bream fTnT gene isoforms afTnTsb (higher molecular mass), LfTnTsb (lower molecular mass) and 18s rRNA assessed by RT-PCR after 7 (64 d.p.h.), 18 (75 d.p.h.) and 31 (89 d.p.h.) days of treatment with T3 and in control animals. Reaction products fractionated on a 2.5% agarose gel (A) and respective graphical representation of the relative expression of isoform afTnTsb (B) and LfTnTsb (C) against 18s; (D) the afTnTsb:LfTnTsb ratio. The efTnTsb isoform is not represented graphically since no expression was detected in any treatment at any sampling point. aSignificant statistical differences between the control and T3-treated groups.





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