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Fig. 3. Genomic organisation of the putative sea bream, Fugu and
Tetraodon fTnT gene predicted after Spidey analysis using the sea
bream fTnT isoform cDNA sequence isolated and the Fugu
scaffold 1617. The same analysis was carried out using the sea bream
fTnT isoforms isolated and Tetraodon scaffold SCAF7217, but
including also the Tetraodon full-length cDNA clones CR660426,
CR658422 and CR657382. Black boxes represent constitutive protein coding
regions whereas the white boxes represent untranslated regions. Broad striped
blocks represent alternatively spliced untranslated exons, while narrow
striped blocks represent protein coding alternatively spliced exons. Exon II
contains the ATG initiation codon (arrowhead) and is composed of part of the
5' UTR and the start of the coding region. The sea bream, Fugu
and Tetraodon fTnT locus has 14 exons from which exon I and XIV are
untranslated exons. The exon numbers with an asterisk are alternatively
spliced exons. Exon V is the larval-specific exon. Flush junction boundaries
in exons indicate that they start or end in intact codons; saw tooth
boundaries indicate that the upstream exon donates one nt to the codon while
the other two are contributed by the downstream exon; concave/convex exon
boundaries indicate that codon splitage takes place by the upstream exon
donating two nt while the downstream exon contributes one. The
efTnTsb isoform results form the incorporation of all exons except
exon I. The afTnTsb isoform results from splicing of exons I-IV and
VI-XIV whereas in the LfTnTsb isoform exons I-III and VI-XIV are
spliced. Both afTnTsb and LfTnTsb have an extra 5' UTR
exon, exon I, which is absent from the efTnTsb
(Fig. 4). Sequence conservation
between this region (exon I) of the presumed adult sea bream fTnT
cDNAs and the genomic sequence of Tetraodon was higher than 88%.
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