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Fig. 4. Unsaturation index and mol % of polyunsaturated fatty acids (PUFA) and
docosahexaenoic acid (22:6n-3) in juvenile hard clams as a function of time,
variety and tidal location (mean ± s.e.m.). Unsaturation index and mol
% of PUFA as a function of time are means of the two clam varieties. The
unsaturation index is calculated as the sum of the mol % of each unsaturated
fatty acid multiplied by the number of double bonds within that fatty acid.
Clams were placed in the field in August 2003 at two tidal locations and
overwintered subtidally and in the laboratory between October 2003 and May
2004. Sampling took place in the field until October (filled symbols) and then
in the laboratory until May (open symbols). Clams were sampled simultaneously
in the field and in the laboratory in May to validate agreement between field
and laboratory results. The first time at which a significant increase
occurred is indicated by a single asterisk.