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Fig. 2. The effect of food and starvation on isothermal tracking. (A)
Representative isothermal tracks of wild-type worms navigating radial thermal
gradients with uniformly spread bacterial lawns. The temperature from center
to edge of the plate was 18.5 to 21.5°C, corresponding to a steepness of
0.7°C cm-1. The time interval between data points is 10 s. (B)
Histogram of the durations of isothermal tracks of wild-type worms navigating
a radial thermal gradient with 0.7°C steepness in the presence of
bacterial lawns. The solid line shows a fit to an exponential
(
=156±6 s; P>0.2). (C) Percentage of time spent in
isothermal movement of wild-type navigating spatial thermal gradients with
0.5°C cm-1 steepness, after overnight cultivation at 20°C
with bacterial food and being placed at 20°C without food for fixed
periods of time. Each data point was quantified by totaling the duration of
each isothermal movement exhibited by 60 worms in 30 min of observation on
linear thermal gradients, and dividing by 1800 min. Error bars represent
± 1 s.e.m.
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