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Fig. 1. Isothermal tracks. (A) Representative isothermal tracks of wild-type worms
(N2 strain) cultivated at 20°C navigating a radial thermal gradient on the
surface of a 9-cm diameter agar plate. The temperature from center to edge is
18.5 to 21.5°C, corresponding to 0.7°C cm-1 steepness. The
trajectory of each isothermal track on the spatial gradient is shown by open
circles connected by black lines, with each circle indicating position of the
worm centroid at 10 s intervals throughout each track. For purposes of
presentation, the undirected movements before and after each isothermal track
are not shown. (B) Histogram of the absolute temperatures of 
400
isothermal tracks made by wild-type worms cultivated at 20°C navigating
linear thermal gradients from 15-25°C. (C) Percentage of time spent in
isothermal movement of wild-type worms navigating defined spatial thermal
gradients, quantified for each data point by totaling the duration of each
isothermal movement exhibited by 60 worms in 30 min of observation on linear
thermal gradients, and then dividing by 1800 min. Error bars represent
± 1 s.e.m. (D) Histogram of the durations of 400 isothermal tracks
made by wild-type worms navigating a radial thermal gradient with 0.7°C
cm-1 steepness. The solid line shows a fit to an exponential
function (
=80 s; P>0.5). (E) Mean durations of isothermal
tracks of wild-type worms navigating radial spatial thermal gradients with
0.2, 0.7, and 1.2°C cm-1 steepness (black circles). Each point
represents data from 400 tracks. For comparison, the mean duration of
1000 runs exhibited by wild-type worms navigating an isotropic
environment at 20°C is also shown (open circle at 0°C
cm-1). Error bars represent ± 1 s.e.m. (F) Percentage of
time spent in isothermal movement of wild-type (WT) and mutant worms
navigating spatial thermal gradients with 0.5°C steepness, quantified for
each data point by totaling the duration of each isothermal movement exhibited
by 60 worms in 30 min of observation on linear thermal gradients, and then
dividing by 1800 min. Error bars represent ± 1 s.e.m.
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