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Figure 4


Fig. 4. Elevated dietary salt intake results in the appearance of seawater-like chloride cells. Representative transmission electron micrographs of gill mitochondria-rich cells (MRC) from (A,B) high-saltfed or (C) control trout. The MRCs can be identified by their abundance of mitochondria (m) and tubular elements. In the high-salt-fed trout, a large mitochondria-rich chloride cell (CC) is flanked by a smaller accessory cell (AC). The apical surfaces of these two adjacent cells form a shallow crypt; this area has been enlarged 2x in B. (C) In the control trout, two large MRCs are shown with microplicae elaborating their apical membranes. Pavement cells (PVC) with less pronounced microplicae are seen between (asterisk) and to the left. This area has been enlarged 2x in the inset. Arrows and arrowheads indicate the MRC-PVC and CC-AC tight junctions, respectively. The lateral membranes of MRCs are indicated by crossed arrows. Scale bars, 1 µm.





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