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Fig. 4. Effect of Ca2+ on trajectories and swimming speed. Trajectories
in the presence or absence of Ca2+ are shown. Sperm trajectories of
Acropora digitifera in Na-free ASW containing (A) 5 mmol
l-1 EGTA and 10 µmol l-1 of the Ca2+
ionophore A23187, (B) 10 mmol l-1 CaCl2 and 10 µmol
l-1 A23187, and (C) 10 mmol l-1 CaCl2. (D)
Swimming speed in each of the solutions. Other species showed similar patterns
(data not shown). Values are mean ± s.d. (N=150 sperm from
five individuals). Scale bars, 50 µm.