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Figure 3


Fig. 3. H2O2 activates endogenous PKC{gamma} enzyme activity in lenses from the control, but not the PKC{gamma} knockout mice. The supernatants from whole lens extracts of control and PKC{gamma} knockout (KO) mice were used to determine PKC{gamma} enzyme activity and protein levels by western blot. (A) Endogenous PKC{gamma} was immunoprecipitated using PKC{gamma} antisera. PKC{gamma} enzyme activity was measured as described in the Materials and methods. Enzyme activity was expressed as a percentage of untreated specific PKC{gamma} activity. Untreated specific PKC{gamma} activity was expressed as 100%. Values are means ± s.e.m. for three independent experiments. The asterisks indicate statistical significance (P≤0.05). (B) Proteins from the supernatants were resolved by SDS-PAGE and immunoblotted with anti-PKC{gamma}, Cx50, Cx43, aquaporin 0 (AQP0) and caveolin 1 (Cav-1). Results demonstrate that the knockout is specific for PKC{gamma}. KO, PKC{gamma} knockout; PBS, phosphate-buffered saline; IB, immunoblot.





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