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Fig. 1. Top view of experimental setup. Two behavioral arenas were cleared in the
seagrass canopy where PVC stakes marked set distances from experimental
shelter openings (25 and 50 cm). Two experimental shelters provided
simultaneous delivery points for either an odorant or a negative (seawater)
control in a modified Y-choice configuration with corresponding shelter
assignments randomized. A submersible infrared camera was mounted on each
shelter's rear end-cap and directed outward. Polyethylene tubing was connected
to the odorant injection and water-sampling ports, with odorants and controls
injected via peristaltic pumps to respective shelters. Baseline
snapper activity was established prior to any odorant delivery. The order of
odorants was randomized for each individual gray snapper to prevent treatment
order effects, and the positive control was always delivered last. Overall
tank flow was directed away from shelters with inflow pipes facing a reef-like
structure, behind which was the tank drain pipe. Gray snappers generally
hovered within the reef structure.
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