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Figure 4


Fig. 4. H2S is produced by homogenates of bovine pulmonary artery and vein and lamprey dorsal aorta. H2S production by pulmonary arteries is inhibited by the CBS inhibitor, amino-oxyacetate (AOA; 1 mmol l–1), the pyridoxyl 5'-phosphate-dependent enzyme inhibitor, hydroxylamine (HA; 1 mmol l–1), but not by the CSE inhibitor D,L-propargylglycine (PPG; 10 mmol l–1); HA also inhibits H2S production in veins. Bovine vessels were pooled from two animals, lamprey aortas were pooled from six fish. Total sulfide (H2S and HS–) was measured in triplicate with ion-selective electrodes after alkaline conversion to S2–. Values are means ± s.e.m.; *significantly different from respective control (P≤0.05).





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