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Fig. 8. Application of crustacean cardioactive peptide (CCAP) results in a
significant increase in the activity of the synaptic endings on the anterior
foreguts of early-molt stage larvae. For these experiments the density of
FM1-43 loaded puncta was used to estimate the endogenous activity of the
presynaptic terminals found on the anterior foregut musculature. (A) Under
control conditions there were relatively few FM1-43-labeled puncta found on
the esophageal dilator muscles of the anterior foregut of an early-molt larva.
(B) However, following a 15 min incubation in 10–8 mol
l–1 CCAP, there is a dramatic increase in the density of
FM1-43-labeled puncta on the muscle. (C) A plot summarizing the FM1-43
results. When loading occurred in control saline the density of FM1-43 puncta
on the early-molt stage anterior foreguts was only about a quarter of the
value calculated for intermolt larvae. Following exposure to
10–8 mol l–1 CCAP for 15 min, the density of
labeled puncta on the anterior foregut muscles of early-molt stage larvae was
similar to that observed in anterior foreguts isolated from intermolt larvae
(P>0.05, unpaired t-test). *Significant difference from
both intermolt saline level (P<0.005, unpaired t-test)
and from the 10–8 mol l–1 CCAP level
(P<0.005, paired t-test). Scale bar, 20 µm.
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