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Figure 3


Fig. 3. The activity-dependent dye FM1-43 reveals that the active hemolymph fraction triggers a decline in the efficacy of the synaptic terminals on the anterior foregut musculature. Presynaptic terminals of intermolt 5th instar larvae were first preloaded with FM1-43 (A,C), and then allowed to unload under one of two conditions: (B) after a continued 20 min incubation in normal physiological saline or, (D) after a 20 min incubation in the hemolymph fraction. (E) In the preparations incubated in the hemolymph fraction, the average percentage change in the fluorescence of the terminals was significantly lower than that of the preparations that had been incubated in saline (unpaired t-test, P<0.01) *Significant difference from the normal saline levels. Scale bar, 50 µm.





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