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Fig. 6. Expression of fast myosin heavy chain (MyHC; A,E), fast myosin binding protein C (B,F), fast myosin light chain 1 (MyLC1; C,G), fast MyLC3 (D,H), and slow MyLC1 (green) and fast MyHC (red) (I–K) in trout larvae. Transverse sections of lateral myotomal muscle at 41 (A–D and I), 48 (J) and 76 (E–H,K) days post-fertilisation. Labelling for fast muscle isoforms is observed in fast muscle fibres and in the superficial layers of slow fibres (arrows, A–H). Merged immunofluorescence images (I–K) show that the most external layers of superficial fibres express both slow and fast muscle isoforms (see arrows showing the overlap of the two fluorescent markers). Scale bars, 40 µm (A–D); 80 µm (E–H); 40 µm (I–J) and 80 µm (K).





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