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Fig. 1. Experimental setup for assessing flight ability of D.
melanogaster. Ten bottles were connected in series to test ten flies
simultaneously. Flies were held for 5 min in the Eppendorf tube to acclimate
to the treatment. The lid of the tube was then removed with a magnet,
releasing the fly into the chamber. We ranked flight performance as one of
three categories: flew above the fluon line within 2 min (`flight'), flew
within an additional 2 min period while the bottle was gently tapped (`coerced
flight'), or failed to fly (`no flight').
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