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Fig. 2. Reverse-phase HPLC of crude salivary gland extract (SGE) of horse fly H. bimaculata. The peaks containing vasodilator activity are indicated by their retention times. The purification procedures are described in the text. (A) Crude SGE purified on a Vydac C4 column; (B) active fraction from A with retention time 13.77 min., second purification on the Beckman Ultrasphere C-18 column; (C) active fraction from B with retention time 14.40 min, third purification on the Vydac C18 column.





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