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Fig. 10. Comparison of regenerate at different times of regeneration and two
different amputation levels (5 and 50 mm; measured as length lost; LL). The
differentiation index (DI; in %) is given below each image. ap, amputation
plane. The first difference was observed on day 6, when the blastema was
longer for the arm amputated at 50 mm from the tip. After 12 days, important
differences in both size and differentiation were observed. The arms cut at 5
mm from the tip regenerated a smaller arm (1.2 mm), which had already started
to differentiate complete segments with spines and tube feet while arms cut at
50 mm from the tip regenerated a longer arm (2 mm) but no complete segments
were formed. These differences were amplified after 19 days. The regenerate
was three times longer for the arm cut at 50 mm from the tip but less
differentiated (DI of 45% versus 70%) than the one cut at 5 mm from
the tip.
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