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Fig. 5. Transcription profiles for crustacean cardioactive peptide (CCAP) mRNA
during the moult cycle of Carcinus (A,C,E) and Callinectes
(B,D,F). Data are shown as un-normalized (copy no. per thoracic ganglion;
A,B), normalized against total RNA (copy no. µg-1 RNA
x106; C,D), and normalized against a housekeeping gene,
arginine kinase (AK; copy no. CCAP mRNAx103/106
copies of AK mRNA; E,F). For Carcinus, N=5-11 independent samples
were taken at each moult stage. For Callinectes, N=8-10. Error bars
indicate +1 s.e.m.