Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig S1. The effect of rapid thermal shifts on ethanol tolerance was strikingly consistent across isofemale lines of D. melanogaster in both populations. Flies reared at 26°C and shifted to 15°C (left pair of bars) had increased ethanol tolerance, whereas flies reared at 15°C and shifted to 26°C (right pair) had decreased tolerance. Data are the inferred LD₅₀ values for each line in each treatment (±95% confidence intervals). There are four line/treatment combinations for which confidence intervals were not estimated owing to poor fit of the probit regression. Line numbers are given in the top right of each panel; numbers only are Innisfail populations; numbers prefixed RK, Tasmania populations.

• Supplemental Figure 2 (Adobe PDF) -

  Fig S2. Mean daily minimum (open symbols) and maximum (closed) temperatures by month in high- and low-latitude locations in Australia, chosen to match those of the collection sites for lines in this experiment. Circles are the difference between daily minimum and maximum temperatures, indicating that daily temperature fluctuations on the order of those used in this experiment (11°C) are environmentally relevant to natural Drosophila populations. Data are from the Australian Government Bureau of Meteorology (http://www.bom.gov.au/).

• Supplemental Tables -

  Adobe PDF