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Fig. 6. Dose- and stage-dependent changes in fictive lung and buccal burst
frequency during application of increasing concentration of the
2-adrenoceptor agonist clonidine (Clo). Clo application was
followed by a 30-90 min wash-out period. (A) Data expressed as a percentage
change from baseline lung values, and (B) data expressed as a percentage
change from baseline buccal values. In A, lung burst frequency responses were
measured in pre-(triangles; N=9) and metamorphic tadpoles (squares;
N=7), and adult frogs (circles; N=12). For fictive buccal
burst frequency responses (B), mean data were obtained in pre-(N=9)
and metamorphic tadpoles (N=6), and adult frogs (N=2).
Adults were excluded from the statistical analysis since the number of
replicates was too low; for this group, data are displayed without error bars.
Values are expressed as means ± s.e.m. *Values statistically
different from baseline, P<0.05. **Values statistically
different from baseline, P<0.10. 
Values
statistically different from corresponding values from the pre-metamorphic
group, P<0.05.
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