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Figure 4


Fig. 4. Relative NHE2 protein expression in control and acidotic fish. (A) This representative western blot, probed with anti-NHE2 antibodies, contains protein from two control (lanes 4 and 8) and two acid-infused (lanes 2 and 6) sculpin. Even-numbered lanes are the membrane fraction and odd-numbered lanes are the cytosolic fraction of homogenized longhorn sculpin gill tissue. An ~85 kDa signal was detected when the immunoblot was probed with anti-NHE2 antibodies (541-AP). Preimmune and peptide-antibody competition controls were negative (not shown). Markers: 100 kDa, 75 kDa. (B) Graph showing differences in relative protein abundance of NHE2 in acid infused versus control fish. Values are mean ratios ± s.e.m. (N=4).





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