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Figure 3


Fig. 3. Colocalization of NHE2, Na+/K+-ATPase and V-ATPase. Staining against both NHE2 (A94-APS: 1/10,000, Vector VIP, purple) and Na+/K+-ATPase ({alpha}5: 1/5000, Vector SG, blue/grey) is evident in the interlamellar region of many gill epithelial cells. No lamellar cells were stained. NHE2 (A94-APS: 1/10,000, Vector VIP, purple) and V-ATPase (HAB: 1/5000, Vector SG, blue/grey) colocalized in some of the same cells. Tissue double-labeled for V-ATPase (HAB: 1/5000, Vector VIP, purple) and Na+/K+-ATPase ({alpha}5: 1/5000, Vector SG, blue/grey) demonstrated diffuse staining of both. Most stained cells only stained for Na+/K+-ATPase, however, some cells stained for both transporters. This could indicate that V-ATPase is present in only a subpopulation of mitochondria-rich cells. *, colocalization in each picture; +, staining of NHE2 only; ^, staining of HAB only; #, staining of Na/K-ATPase only.





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