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Figure 1


Fig. 1. Longhorn sculpin gill sections from seawater controls with chromagenic and fluorescent staining against NHE2 and Na+/K+-ATPase. Gill sections stained with (A) DAB and (B) green fluorescein against NHE2 with sculpin-specific antibodies. Controls (lacking primary antibody, antigen-antibody competition and pre-immune serum) were all negative (not shown). Staining is punctate in the apical and subapical regions of large cells in the filament. The cell distribution is similar to the pattern for Na+/K+-ATPase-rich cells. Serial sections stained with (C) DAB and (D) red-rhodamine against Na+/K+-ATPase show intense immunoreactivity throughout the cell which is consistent with staining along the tubular infoldings of the basolateral membrane in cells that are probably mitochondria-rich chloride cells. Scale bars, 20 µm.





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