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Figure 8


Fig. 7. Eight candidates label cells exclusively outside of the CNS. Panels show representative sections of a variety of tissues labelled using in situ hybridization, and are organized vertically by type of tissue labelled: (A-D) alimentary canal, (E-G) male reproductive system, (H-J) female reproductive system, and (K,L) other tissues. Frontal sections through the gut demonstrate that the riboprobe for CG3252 (A) and CG15279 (B) both label cells in the cardia (car; *), a structure at the transition between the foregut and the midgut. Ventral nerve cord is marked (vnc). (C,D) The anti-sense riboprobe for CG8850 (C) and CG1698 (D) both label ventricular cells (vc; filled arrows) that line the alimentary canal (ac) that runs from the thorax (th) into the abdomen. CG8850 labels cells in a restricted domain of ventricular cells in the abdomen; neighbouring ventricular cells along the alimentary canal are not labelled. Sagittal sections reveal that CG7075 (E), CG1698 (F) and CG8291 (G) label male reproductive tissue, likely the testis (tes) in the male abdomen (arrows). In addition, CG8291 (G) labels the rectal bulb (rb; open arrows), a hindgut structure at the end of the alimentary canal. Insets show boxed areas at higher magnification. Horizontal (H) and sagittal (I,J) sections show that CG4476 (H), CG1698 (I), and CG8291 (J) label structures in the female reproductive system. CG4476 (H) and CG8291 (I) label nurse cells (nc; arrows) and developing oocytes (oo; *). CG1698 (I) labels a structure called the calyx (cal; arrows). (K) CG15279 (mt; arrows) also labels two closely apposed Malpighian tubules. (L) Midgut precursor cells (mgp; arrowhead) and the Garland cells (gc; arrows) were labelled by CG13795 in a stage 14 embryo. At later stages, only the Garland cells are labelled. Anterior of the embryo is in the upper left corner and this is a dorsal view of the embryo. Scale bars, 50 µm (A,B), 100 µm (C,F,I-K) and 200 µm (D,E,G,H). A, anterior; D, dorsal.





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