|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. CG1732 and CG15088 are expressed in a glial-like pattern.
The various glial subtypes are shown in (B) and the corresponding area in a
sequential section is shown in (A). Examples of perineural (arrow) and
subperineural (carrot) glia are marked [nomenclature according to Eule et al.
(Eule et al., 1995)]. Inner
chiasmatic glia are marked by the barbed arrow. Neuropil are labelled
Medulla/Lobula (Me/Lo), suboesophageal ganglion (SEG), central brain (CB), and
photoreceptors (PR). (A) Frontal section of the fly head showing the brain and
optic lobes. Cells labelled with the anti-sense riboprobe for CG1732
surround the neuropils of both structures. The label occurs in regularly
spaced intervals; the neuropils have a purple hue suggesting that the mRNA may
be present in penetrating processes. (B) Sequential section to A labelled with
the glial marker, Repo. Cells that express CG1732 are likely to be
glia because they occupy the same anatomical position and approximate spacing
as cells that label with the nuclear glia marker Repo. Boxed areas in A and B
denote areas shown at higher magnification in C and D, respectively. (C)
Higher magnification of the optic lobes in A. In both C and D, arrowheads
indicate labelled cells in either outer chiasm giant glia or marginal glia at
the proximal margin of the lamina neuropil. Medulla neuropil (MeN), Medulla
cell bodies (MeCB), and lamina neuropil (LaN) are denoted. Arrows show the
location of glia at the distal margin of the lamina; open arrows show position
occupied by epithelial glia. Notched arrows mark positions of medulla neuropil
glia. Glia distal to the lamina neuropil do not label with CG1732 and
epithelial glia at the distal margin may faintly label for CG1732.
(D) Higher magnification of B showing Repo label in the large glial nuclei of
the outer chiasm glia (arrowheads) at the proximal margin of the lamina.
Arrows show glia at the distal border of the lamina and open arrows show the
epithelial glia; both label for Repo. Notched arrows mark the nuclei of
medulla neuropil glia. (E) Pattern of label of CG15088 probe. (F) the
Repo antibody labels nuclei in similar sections from different preparations.
Asterisks denote comparable neuropils. Like Repo, CG15088 appears to
label glia throughout the cortex. All sections are frontal sections with
dorsal up; scale bars, 200 µm (A,B,E,F), 50 µm (C,D).
|
