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Figure 4


Fig. 3. The segmental mRNA expression profile of the SLC6 homologs in the head, thorax and abdomen of both male and female flies determined by RT-PCR. The head (H), thorax (T) and abdomen (A) were isolated from either male or female flies and RT-PCR was performed to determine presence or absence of transcript in a given body segment. Candidates are arranged from top to bottom as: (i) those expressed differentially in the three segments; (ii) those showing gender specificity; (iii) those expressed equally in the segments; (iv) control genes HDC and rp49. HDC is known to have enriched expression in the head and thorax and rp49 controlled for equivalent starting template in the PCR reactions. The column labelled `No RT' showed controls confirming that the amplicons were dependent on the presence of cDNA. Band size is measured in base pairs (bp).





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