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Fig. 2. Phylogenetic analysis assigns many Drosophila SLC6 proteins to
previously identified subfamilies and a novel IAAT subfamily. An unrooted
phylogenetic tree displaying Drosophila candidate amino acid (AA)
sequences with cloned and predicted SLC6 transporter amino acid sequences from
multiple organisms. Candidate Drosophila SLC6 transporters are
labelled in bold and underlined. Prominently represented among the insect
sequences are putative SLC6s from Anopheles gambiae (Ag), a mosquito
known for carrying the malaria parasite and whose genome sequence was recently
reported (Holt et al., 2002).
We also have included known transporter sequences from insects such as the
cabbage looper, Trichoplusia ni (Tn), the tobacco caterpillar
Manduca sexta (Ms) and from mammals. See Table S1 in supplementary
material for a list of the sequence names, abbreviations and accession
numbers. Subfamilies (monoamine transporters, GABA transporters, orphan
neurotransmitter transporters, insect amino acid transporters) are indicated.
As expected, a number of sequences do not group with the previously identified
subfamilies. Many of the C. elegans transporters are quite divergent
and group with each other; likewise, the prokaryote transporters are most
similar to each other but do not segregate into a recognized subfamily with
eukaryote transporters. Several of the candidate Drosophila
transporters form a small subgroup that does not convincingly group with any
other transporters. Nodes that are identical in >90% of bootstrap trials
are denoted by filled circles and nodes that are identical in 75-90% of
bootstrap trials are denoted by empty circles. The complete multiple sequence
alignment from which this phylogenetic tree was generated is available as Fig.
S1 in supplementary material.
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