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Fig. 7. Force-length relationship using the in vivo stimulation pattern. Representative force-length relationships for muscles 178 (A) and 179 (B). The force-length relationships at all three different stimulation levels were obtained from some individuals. (C) Forces normalized to the 3s force at the optimal length determined from the tetanic force-length relationships of muscles 178 (solid blue symbols) and 179 (open red symbols). Forces were normalized to the force at the optimal lengths determined from the tetanic force-length relationships (8.5% strain for muscle 178 and 10.9% strain for muscle 179). Fourth-order polynomial curves were fit to the combined data for muscle 178 (bold, solid blue line; R²=0.95) and muscle 179 (red, broken line; R²=0.96). N=4 animals for each muscle. The bold, black lines represent the difference in isometric force generated by the two muscles at -8% strain, which both muscles experience in vivo (see Results).

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