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Figure 1


Fig. 1. Ostf1 mRNA response to hyperosmotic stress and dexamethasone (DEX) in EGCPC. (A) Primary cultures were exposed to isosmotic medium or different hyperosmotic media in the absence (black bars) or with the addition of 1 µmol l-1 DEX (grey bars). After 2 h treatment, cells were collected and mRNA quantified by qPCR. Relative Ostf1 mRNA abundances with respect to control (isosmotic medium, no DEX) and normalized to ß-actin content are shown. Values are means ± s.e.m. (N=3). Asterisks indicate significant differences (P<0.05) relative to the control. (B) Primary cultures were exposed to isosmotic medium (open squares) or 600 mOsm kg-1 hyperosmotic medium (black triangles) prepared by addition of NaCl. At the indicated times cells were collected and mRNA quantified by qPCR. Relative Ostf1 mRNA abundances with respect to control (time zero) and normalized to ß-actin content are shown. Values are means ± s.e.m. Asterisks indicate significant differences (P<0.05) relative to the control. N=3.





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