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Fig. 7. Co-localization of crustacean hyperglycemic hormone (CHH)-like and CHH
precursor-related peptide (CPRP)-like immunoreactivity in the pericardial
organ (PO) of Cancer productus. This series of micrographs consists
of pseudo-colored single optical sections collected from a single focal plane
from a PO labeled with both anti-CPRP (A, pseudo-colored green) and anti-CHH
(B, pseudo-colored red). When A and B are merged (C), complete overlap in the
labeled structures was revealed (i.e. yellow, but not green or red, coloration
is seen in all immunopositive processes in the micrograph), suggesting that
the CPRP- and CHH-like labels are contained in a common set of structures in
the PO. A and B are shown at the same magnification. Scale bars in B and C, 25
µm.
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